Year
2018
Degree Name
PhD thesis
Department
School of Chemistry
Abstract
Cellular DNA is constantly under threat from exogenous as well as endogenous genotoxic agents that result in DNA damage. Transcription, the first step of gene expression, helps to safeguard the genome via the transcription-coupled repair (TCR) process. During this reaction, transcription complexes stalled at sites of lesions stimulate recruitment of DNA repair factors. In prokaryotes, TCR is mediated by the translocase Mfd. Mfd displaces stalled transcription complexes and subsequently recruits UvrA-UvrB, the damage recognition complex that detects bulky lesions. How TCR is orchestrated in cellular environments remains poorly understood. To further our understanding of TCR in live cells, we developed fluorescent probes to visualise single molecules of Mfd, UvrA and UvrB in the model organism Escherichia coli.
Recommended Citation
Ho, Han Ngoc, Visualisation of transcription-coupled repair in live Escherichia coli, PhD thesis thesis, School of Chemistry, University of Wollongong, 2018. https://ro.uow.edu.au/theses1/742
FoR codes (2008)
029901 Biological Physics, 060107 Enzymes, 060407 Genome Structure and Regulation, 0601 BIOCHEMISTRY AND CELL BIOLOGY
Unless otherwise indicated, the views expressed in this thesis are those of the author and do not necessarily represent the views of the University of Wollongong.