Year
1994
Degree Name
Master of Science (Hons.)
Department
Department of Biology
Recommended Citation
Tsai, Mingjui, Application of the random amplified polymorphic DNA (RAPD) technique to the identification and classification of Dunaliella species, Master of Science (Hons.) thesis, Department of Biology, University of Wollongong, 1994. https://ro.uow.edu.au/theses/2754
Abstract
Dumliella is a genus of green microalga. It is an important organism for the study of osmoregulation and is cultured for the production of p-carotene. The classification of Dumliella species is not clear, especially because the morphological characters are changeable under different culture conditions. As a first step to providing an improved method for identification and classification, a DNA fingerprinting method called Random Amplified Polymorphic DNA (RAPD) assay was applied to three Dimaliella species, D. tertiolecta, D. salina, and D. viridis. A method for extraction of DNA from DunaRella was developed and the parameters that determine the RAPD pattern were assessed. RAPD patterns specific to each of the three Dimaliella species were demonstrated.
Unless otherwise indicated, the views expressed in this thesis are those of the author and do not necessarily represent the views of the University of Wollongong.