Elucidating the Stoichiometries of Host–Pathogen Protein Interactions with Mass Photometry
Publication Name
Methods in Molecular Biology
Abstract
Mass photometry (MP) is a single molecule technique that enables the characterization of individual proteins. Here we show a detailed workflow using the Refeyn OneMP to investigate molecular complexes, using the M53 protein, a plasminogen-binding group A streptococcal M-like protein (PAM), and human plasminogen as exemplar proteins. The methodology described herein confirmed a 1:1 binding stoichiometry for the M53–plasminogen complex. Additionally, MP was used to identify the oligomerization state, homogeneity, purity, and approximate molecular weights of each protein.
Open Access Status
This publication is not available as open access
Volume
2674
First Page
201
Last Page
208