Title

Pixel reassignment in image scanning microscopy with a doughnut beam: Example of maximum likelihood restoration

Publication Name

Journal of the Optical Society of America A: Optics and Image Science, and Vision

Abstract

In image scanning microscopy, the pinhole of a confocal microscope is replaced by a detector array. The point spread function for each detector element can be interpreted as the probability density function of the signal, the peak giving the most likely origin. This thus allows a formof maximum likelihood restoration, and compensation for aberrations, with similarities to adaptive optics. As an example of an aberration, we investigate theoretically and experimentally illumination with a vortex doughnut beam. After reassignment and summation over the detector array, the point spread function is compact, and the resolution and signal level higher than in a conventional microscope.

Open Access Status

This publication is not available as open access

Volume

38

Issue

7

First Page

1075

Last Page

1084

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Link to publisher version (DOI)

http://dx.doi.org/10.1364/JOSAA.426473