Pixel reassignment in image scanning microscopy with a doughnut beam: Example of maximum likelihood restoration
Publication Name
Journal of the Optical Society of America A: Optics and Image Science, and Vision
Abstract
In image scanning microscopy, the pinhole of a confocal microscope is replaced by a detector array. The point spread function for each detector element can be interpreted as the probability density function of the signal, the peak giving the most likely origin. This thus allows a formof maximum likelihood restoration, and compensation for aberrations, with similarities to adaptive optics. As an example of an aberration, we investigate theoretically and experimentally illumination with a vortex doughnut beam. After reassignment and summation over the detector array, the point spread function is compact, and the resolution and signal level higher than in a conventional microscope.
Open Access Status
This publication is not available as open access
Volume
38
Issue
7
First Page
1075
Last Page
1084