Development of an UHPLC-MS/MS method for remifentanil quantification in a small plasma volume

Authors

Kate O'Hara, University of Newcastle
Jennifer J. Schneider, University of Newcastle
Alison L. Jones, University of WollongongFollow
Ian M. R Wright, University of WollongongFollow
Jennifer H. Martin, University of NewcastleFollow
Peter Galettis, University of Newcastle

RIS ID

137376

Publication Details

O'Hara, K., Schneider, J. J., Jones, A. L., Wright, I. M. R., Martin, J. H. & Galettis, P. (2019). Development of an UHPLC-MS/MS method for remifentanil quantification in a small plasma volume. Journal of Liquid Chromatography and Related Technologies, 42 (15-16), 521-527.

Abstract

Remifentanil is a short acting opioid currently used in anesthesia and as an analgesic. This paper describes a simple, fast HPLC-MS/MS methodology that allows detection of remifentanil in low volume plasma samples. Acetonitrile protein precipitation is used for sample extraction and clean up. The assay has a lower limit of detection of 0.25 ng/mL and a 3 min run time.