Using yeast hybrid system to identify proteins binding to small molecules

Authors

You Wang, University of WollongongFollow
David S. Letham, Australian National University
Peter C. L John, Australian National University
Ren Zhang, University of WollongongFollow

RIS ID

128347

Publication Details

Wang, Y., Letham, D. S., John, P. C. L. & Zhang, R. (2018). Using yeast hybrid system to identify proteins binding to small molecules. In L. Onate-Sanchez (Ed.), Two-Hybrid Systems: Methods and Protocols (pp. 225-234). New York: Humana Press.

Abstract

Protein-small molecule interaction studies provide useful insights into biological processes taking place within the living cell. A special yeast hybrid system, the yeast three-hybrid method, has been developed and used to explore proteins that bind to small molecules, by which means it may be possible to unravel biological processes and dissect function of biological systems. Here we present a protocol employing this method for identifying such binding proteins.