RIS ID
141278
Abstract
The historical development of multiphoton microscopy is described, starting with a review of two-photon absorption, and including two- and three-photon fluorescence microscopies, and second- and third-harmonic generation microscopies. The effects of pulse length on signal strength and breakdown are considered. Different contrast mechanisms, including use of nanoparticles, are discussed. Two new promising techniques that can be applied to multiphoton microscopy are described.
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Publication Details
Sheppard, C. (2020). Multiphoton microscopy: a personal historical review, with some future predictions. Journal of Biomedical Optics, 25 (1), 1-11.