P2X7 receptor activation causes phosphatidylserine exposure in canine erythrocytes

Authors

Megan Faulks, University of Wollongong
Tracey A. Kuit, University of WollongongFollow
Reece Sophocleous, University of WollongongFollow
Belinda L. Curtis, Albion Park Veterinary Hospital
Stephen J. Curtis, Albion Park Veterinary Hospital
Lisa M. Jurak, University of Wollongong
Ronald Sluyter, University of WollongongFollow

RIS ID

110183

Publication Details

Faulks, M., Kuit, T. A., Sophocleous, R. A., Curtis, B. L., Curtis, S. J., Jurak, L. M. & Sluyter, R. (2016). P2X7 receptor activation causes phosphatidylserine exposure in canine erythrocytes. World Journal of Hematology, 5 (4), 88-93.

Abstract

AIM To determine if activation of the ATP-gated P2X7 receptor channel induces phosphatidylserine (PS) exposure in erythrocytes from multiple dog breeds. METHODS Peripheral blood was collected from 25 dogs representing 13 pedigrees and seven crossbreeds. ATP-induced PS exposure on canine erythrocytes in vitro was assessed using a flow cytometric Annexin V binding assay. RESULTS ATP induced PS exposure in erythrocytes from all dogs studied. ATP caused PS exposure in a concentrationdependent manner with an EC50 value of 395 μmol/L. The non-P2X7 agonists, ADP or AMP, did not cause PS exposure. The P2X7 antagonist, AZ10606120, but not the P2X1 antagonist, NF449, blocked ATP-induced PS exposure. CONCLUSION The results indicate that ATP induces PS exposure in erythrocytes from various dog breeds and that this process is mediated by P2X7 activation.