Elucidation of markers for monitoring morphine and its analogs in urine adulterated with pyridinium chlorochromate

Authors

Susan Luong, University of Technology, SydneyFollow
Unnikrishnan Kuzhiumparambil, University of Technology, Sydney
Shanlin Fu, University of Technology, SydneyFollow

RIS ID

104698

Publication Details

Luong, S., Kuzhiumparambil, U. & Fu, S. (2015). Elucidation of markers for monitoring morphine and its analogs in urine adulterated with pyridinium chlorochromate. Bioanalysis, 7 (18), 2283-2295.

Abstract

Aim: Currently, procedures that identify the drugs 'destroyed' in adulterated urine specimens are very limited. This study aimed to determine the effect of pyridinium chlorochromate (PCC) on routine opiate assays and identify reaction products formed. Results/methodology: Opiate-positive urines adulterated with PCC (20 and 100 mM) were analyzed using CEDIA® immunoassay and GC-MS. Urine and water samples spiked with 6-monoacetylmorphine, morphine and its glucuronides (10 µg/ml) and PCC (0.02-100 mM) were monitored with LC-MS, and the products characterized. Conclusion: PCC significantly decreased the abundance of morphine, codeine and IS. Adulterated water and urine samples containing 6-monoacetylmorphine, morphine and morphine-3-glucuronide yielded morphinone-3-glucuronide, 7,14-dihydroxy-6-monoacetylmorphine, 7,8-diketo-6-monoacetylmorphine and 7,8-diketo-morphine (tentative assignment). Reaction pathways may be different in the two matrices.