Probing molecular choreography through single-molecule biochemistry

Authors

Antoine M. van Oijen, University of WollongongFollow
Nicholas E. Dixon, University of WollongongFollow

RIS ID

104419

Publication Details

van Oijen, A. M. & Dixon, N. E. (2015). Probing molecular choreography through single-molecule biochemistry. Nature Structural and Molecular Biology, 22 (12), 948-952.

Abstract

Single-molecule approaches are having a dramatic impact on views of how proteins work. The ability to observe molecular properties at the single-molecule level allows characterization of subpopulations and acquisition of detailed kinetic information that would otherwise be hidden in the averaging over an ensemble of molecules. In this Perspective, we discuss how such approaches have successfully been applied to in vitro-reconstituted systems of increasing complexity.