Graphene oxide absorbed anti-IL10R antibodies enhance LPS induced immune responses in vitro and in vivo

Authors

Guoying Ni, University of WollongongFollow
Yuejian Wang, Health Supervision Institute, Hebei Province, China
Xiaolian Wu, The First People'S Hospital of Foshan
Xiongfei Wang, The First People'S Hospital of Foshan
Shu Cheng, The First People'S Hospital of FoshanFollow
Xiaosong Liu, University of WollongongFollow

RIS ID

75809

Publication Details

Ni, G., Wang, Y., Wu, X., Wang, X., Cheng, S. & Liu, X. (2012). Graphene oxide absorbed anti-IL10R antibodies enhance LPS induced immune responses in vitro and in vivo. Immunology Letters, 148 (2), 126-132.

Abstract

Interleukin 10 is an anti-inflammatory cytokine which limits immune responses to both self and foreign antigens. Blocking IL10 at the time of immunization increases cytotoxic T cell responses in antigen experienced host, a situation similar to therapeutic vaccination for cancer and chronic viral infection, where patients usually develop ineffective immune responses to tumour or viral antigens before immunotherapy starts. Graphene oxide (GO) is a nano material often used for drug delivery and tissue engineering. In the current paper, we demonstrated that GO is able to absorb anti-IL10 receptor antibodies. The anti-IL10R antibodies absorbed in GO are slowly released and the release of absorbed antibodies is pH dependent. GO absorbed anti-IL10R antibodies are bioactive both in vitro and in vivo. GO absorbed anti-IL10R antibodies are more efficient than free anti-IL10R antibodies at eliciting LPS stimulated CD8 T cell responses. Our results suggest that GO is able to absorb anti-IL10R antibodies and absorbed anti-IL10R antibody may be useful as an adjuvant for vaccination and ideal for delivering to tumour site, and breaking of suppressive tumour environment.