Characterisation of sphingolipids in the human lens by thin layer chromatography-desorption electrospray ionisation mass spectrometry

Authors

Jo Ann Seng, University of WollongongFollow
Shane R. Ellis, University of WollongongFollow
Jessica Hughes, University of WollongongFollow
Alan Maccarone, University of WollongongFollow
Roger J. W Truscott, University of WollongongFollow
Stephen J. Blanksby, University of WollongongFollow
Todd W. Mitchell, University of WollongongFollow

RIS ID

91792

Publication Details

Seng, J., Ellis, S. R., Hughes, J. R., Maccarone, A. T., Truscott, R. J., Blanksby, S. J. & Mitchell, T. W. (2014). Characterisation of sphingolipids in the human lens by thin layer chromatography-desorption electrospray ionisation mass spectrometry. BBA: Molecular and Cell Biology of Lipids, 1841 (9), 1285-1291.

Abstract

The lipidome of the human lens is unique in that cholesterol and dihydrosphingomyelin are the dominant classes. Moreover, the lens lipidome is not static with dramatic changes in several sphingolipid classes associated with both aging and cataract. Accordingly, there is a clear need to expand knowledge of the molecular species that constitute the human lens sphingolipidome. In this study, human lens lipids have been extracted and separated by thin-layer chromatography (TLC). Direct analysis of the TLC plates by desorption electrospray ionisation-mass spectrometry (DESI-MS) allowed the detection over 30 species from 11 classes of sphingolipids. Significantly, novel classes of lens lipids including sulfatides, dihydrosulfatides, lactosylceramide sulfates and dihydrolactosylceramide sulfates were identified.

Grant Number

ARC/CE0561607, ARC/FT110100249, ARC/DP120102922

Additional Grant Number

http://purl.org/au-research/grants/arc/FT110100249

http://purl.org/au-research/grants/arc/DP120102922

http://purl.org/au-research/grants/arc/CE0561607