Efficient generation of mouse ESCs-like pig induced pluripotent stem cells

Authors

Qi Gu, University of WollongongFollow
Jie Hao, Chinese Academy of Sciences, Beijing, China
Tang Hai, Chinese Academy of Sciences, Beijing, China
Jianyu Wang, Chinese Academy of Sciences, Beijing, China
Yundan Jia, Chinese Academy of Sciences, Beijing, China
Qingran Kong, Northeast Agricultural University of China
Juan Wang, Northeast Agricultural University of China
Chunjing Feng, Chinese Academy of Sciences, Beijing, China
Binghua Xue, Northeast Agricultural University of China
Bingteng Xie, Northeast Agricultural University of China
Shichao Liu, Northeast Agricultural University of China
Jinyu Li, Northeast Agricultural University of China
Yilong He, Northeast Agricultural University of China
Jialu Sun, Northeast Agricultural University of China
Lei Liu, Chinese Academy of Sciences, Beijing, China
Liu Wang, Chinese Academy of Sciences, Beijing, China
Zhonghua Liu, Northeast Agricultural University of China
Qi Zhou, Chinese Academy of Sciences, Beijing, China

RIS ID

90375

Publication Details

Gu, Q., Hao, J., Hai, T., Wang, J., Jia, Y., Kong, Q., Wang, J., Feng, C., Xue, B., Xie, B., Liu, S., Li, J., He, Y., Sun, J., Liu, L., Wang, L., Liu, Z. & Zhou, Q. (2014). Efficient generation of mouse ESCs-like pig induced pluripotent stem cells. Protein & Cell, 5 (5), 338-342.

Abstract

Dear Editor, Porcine induced pluripotency stem cells (piPSCs) are promised in basic research, animal husbandry and regenerative medicine. However, the efficiency of the piPSCs induction has been low and the generated piPSCs varied in cell morphology and cell characteristics. Here we report a novel approach to improve efficiency of piPSCs generation. The induced piPSCs are dome-shaped mouse embryonic stem cells (ESCs)-like and display molecular properties of mouse ESCs. Electroporation study reveals that mouse ESCs-like status facilitates genetic manipulating of piPSCs. Importantly, we demonstrate that the domed piPSC colonies are more suitable as donor cells for nuclear transfer (NT) to generate reconstructed embryos than those flattened piPSCs. The potential applications of the newly generated piPSCs in ungulate pluripotent research are discussed.