Daptomycin in vitro activity against methicillin-resistant staphylococcus aureus is enhanced by D-cycloserine in a mechanism associated with a decrease in cell surface charge

Authors

O Gasch, Beth Israel Deaconess Medical Center
S K. Pillai, Beth Israel Deaconess Medical Center
J Dakos, Beth Israel Deaconess Medical Center
Spiros Miyakis, University of WollongongFollow
R C. Moellering, Beth Israel Deaconess Medical Center
G M. Eliopoulos, Beth Israel Deaconess Medical Center

RIS ID

82056

Publication Details

Gasch, O., Pillai, S. K., Dakos, J., Miyakis, S., Moellering, R. C. & Eliopoulos, G. M. (2013). Daptomycin in vitro activity against methicillin-resistant staphylococcus aureus is enhanced by D-cycloserine in a mechanism associated with a decrease in cell surface charge. Antimicrobial Agents and Chemotherapy, 57 (9), 4537-4539.

Abstract

The killing activity of daptomycin against an isogenic pair of daptomycin-susceptible and daptomycin-nonsusceptible (DNS) methicillin-resistant Staphylococcus aureus (MRSA) strains was enhanced by the addition of certain cell wall agents at 1× MIC. However, when high inocula of the DNS strain were used, no significant killing was observed in our experiments. Cytochrome c binding assays revealed d-cycloserine as the only agent associated with a reduction in the cell surface charge for both strains at the concentrations used.