Green fluorescent protein-based biosensor to detect and quantify stress responses induced by DNA-degrading colicins

Authors

Sam Abraham, University of WollongongFollow
James Chin, Elizabeth Macarthur Agricultural Institute
Huub J. M. Brouwers, Elizabeth Macarthur Agricultural Institute
Bernadette Turner, Elizabeth Macarthur Agricultural Institute
Ren Zhang, University of WollongongFollow
Toni A. Chapman, Elizabeth Macarthur Agricultural Institute

RIS ID

38671

Publication Details

Abraham, S., Chin, J., Brouwers, H. J.M.., Turner, B., Zhang, R. & Chapman, T. A.. (2011). Green fluorescent protein-based biosensor to detect and quantify stress responses induced by DNA-degrading colicins. Applied and Environmental Microbiology, 77 (18), 6691-6693.

Abstract

Here we report the development of a whole-cell biosensor to detect and quantify the induction of the SOS response activated by DNA-degrading colicins. This biosensor utilizes the SOS-responsive cda promoter to regulate the expression of green fluorescent protein. The biosensor assay revealed induction of stress for all DNA-degrading reference colicins (E2, E7, and E8).