RIS ID
35338
Abstract
3-Chloro-Abu and 4-chloro-Nva are biosynthetically incorporated into E. coli peptidyl-Pro cis-trans isomerase B, as substitutes for Val and Leu, respectively. The extent of incorporation is up to 90%, and substituted protein is catalytically active. By contrast, 4-chloro-Val is not an effective replacement for Ile.
Included in
Life Sciences Commons, Physical Sciences and Mathematics Commons, Social and Behavioral Sciences Commons
Publication Details
Stigers, D. J., Watts, Z. I., Hennessy, J. E., Kim, H., Martini, R., Taylor, M. C., Ozawa, K., Keillor, J. W., Dixon, N. E. & Easton, C. (2011). Incorporation of chlorinated analogues of aliphatic amino acids during cell-free protein synthesis. Chemical Communications, 47 (6), 1839-1841.