Direct lipid profiling of single cells from inkjet printed microarrays

Authors

Shane Ellis, University of WollongongFollow
Cameron Ferris, University of WollongongFollow
Kerry J. Gilmore, University of WollongongFollow
Todd W. Mitchell, University of WollongongFollow
Stephen J. Blanksby, University of WollongongFollow
Marc in het Panhuis, University of WollongongFollow

RIS ID

72499

Publication Details

Ellis, S., Ferris, C., Gilmore, K. J., Mitchell, T. W., Blanksby, S. J. & in het Panhuis, M. (2012). Direct Lipid Profiling of Single Cells from Inkjet Printed Microarrays. Analytical Chemistry, 84 (22), 9679-9683.

Abstract

The on-demand printing of living cells using inkjet technologies has recently been demonstrated and allows for the controlled deposition of cells in microarrays. Here, we show that such arrays can be interrogated directly by robot-controlled liquid microextraction coupled with chip-based nanoelectospray mass spectrometry. Such automated analyses generate a profile of abundant membrane lipids that are characteristic of cell type. Significantly, the spatial control in both deposition and extraction steps combined with the sensitivity of the mass spectrometric detection allows for robust molecular profiling of individual cells.