Rapid flow cytometric measurement of protein inclusions and nuclear trafficking

Authors

Daniel Whiten, University of WollongongFollow
Rebecca San Gil, University of WollongongFollow
Luke McAlary, University of WollongongFollow
Justin J. Yerbury, University of WollongongFollow
Heath Ecroyd, University of WollongongFollow
Mark R. Wilson, University of WollongongFollow

RIS ID

109219

Publication Details

Whiten, D. R., San Gil, R., McAlary, L., Yerbury, J. J., Ecroyd, H. & Wilson, M. R. (2016). Rapid flow cytometric measurement of protein inclusions and nuclear trafficking. Scientific Reports, 6 31138-1 - 31138-9.

Abstract

Proteinaceous cytoplasmic inclusions are an indicator of dysfunction in normal cellular proteostasis and a hallmark of many neurodegenerative diseases. We describe a simple and rapid new flow cytometry-based method to enumerate, characterise and, if desired, physically recover protein inclusions from cells. This technique can analyse and resolve a broad variety of inclusions differing in both size and protein composition, making it applicable to essentially any model of intracellular protein aggregation. The method also allows rapid quantification of the nuclear trafficking of fluorescently labelled molecules.