Generation of Neural Crest Progenitors from Human Pluripotent Stem Cells

Authors

Kwaku Dad Abu-Bonsrah, Murdoch Children's Research Institute, University of Melbourne
Serena Viventi, University of Melbourne
Donald F. Newgreen, Royal Children's Hospital, Murdoch Children's Research Institute
Mirella Dottori, University of Melbourne, University of WollongongFollow

RIS ID

135297

Publication Details

Abu-Bonsrah, K., Viventi, S., Newgreen, D. F. & Dottori, M. (2019). Generation of Neural Crest Progenitors from Human Pluripotent Stem Cells. Methods in Molecular Biology, 1976 37-47.

Abstract

There are a vast range of diseases and disorders that are neurocristopathic in origin, including Hirschsprung's disease, pheochromocytoma, familial dysautonomia, craniofacial disorders, and melanomas. Having a source of human neural crest cells is highly valuable for investigating potential treatments for such diseases. This chapter describes a robust and well-characterized protocol for deriving neural crest from human pluripotent stem cells (hPSCs), which can then be differentiated to neuronal and non-neuronal lineages. The protocol is adapted to suit hPSC maintenance as a monolayer bulk culture or as manual-passaged colonies, which makes it widely applicable to researchers that may use different systems for hPSC maintenance.