Design of DNA rolling-circle templates with controlled fork topology to study mechanisms of DNA replication

Authors

Enrico Monachino, University of Wollongong, University of GroningenFollow
Harshad Ghodke, University of WollongongFollow
Richard R. Spinks, University of WollongongFollow
Ben Hoatson, University of Wollongong
Slobodan Jergic, University of WollongongFollow
Zhi-Qiang Xu, University of WollongongFollow
Nicholas E. Dixon, University of WollongongFollow
Antoine M. van Oijen, University of WollongongFollow

RIS ID

129066

Publication Details

Monachino, E., Ghodke, H., Spinks, R. R., Hoatson, B. S., Jergic, S., Xu, Z., Dixon, N. E. & van Oijen, A. M. (2018). Design of DNA rolling-circle templates with controlled fork topology to study mechanisms of DNA replication. Analytical Biochemistry, 557 42-45.

Abstract

Rolling-circle DNA amplification is a powerful tool employed in biotechnology to produce large from small amounts of DNA. This mode of DNA replication proceeds via a DNA topology that resembles a replication fork, thus also providing experimental access to the molecular mechanisms of DNA replication. However, conventional templates do not allow controlled access to multiple fork topologies, which is an important factor in mechanistic studies. Here we present the design and production of a rolling-circle substrate with a tunable length of both the gap and the overhang, and we show its application to the bacterial DNA-replication reaction.

Grant Number

ARC/DP180100858

Grant Number

ARC/DP150100956

Additional Grant Number

http://purl.org/au-research/grants/ARC/FL140100027