Peptide grafted and self-assembled poly(γ-glutamic acid)-phenylalanine nanoparticles targeting camptothecin to glioma

Authors

Hitesh Kulhari, RMIT University, CSIR-Indian Institute of Chemical Technology
Srinivasa Telukutla, RMIT University, CSIR-Indian Institute of Chemical Technology
Deep Pooja, CSIR-Indian Institute of Chemical Technology
Ravi Shukla, RMIT University
Ramakrishna Sistla, CSIR-Indian Institute of Chemical Technology
Vipul Bansal, RMIT University
David J. Adams, University of WollongongFollow

RIS ID

115403

Publication Details

Kulhari, H., Telukutla, S. R., Pooja, D., Shukla, R., Sistla, R., Bansal, V. & Adams, D. J. (2017). Peptide grafted and self-assembled poly(γ-glutamic acid)-phenylalanine nanoparticles targeting camptothecin to glioma. Nanomedicine, 12 (14), 1661-1674.

Abstract

Aim: To synthesize cRGDfK peptide conjugated poly(γ-glutamic acid)-phenylalanine nanoparticles to improve the therapeutic efficacy of camptothecin (CPT) against glioblastoma multiforme.

Methods: Peptide-conjugated, drug-loaded nanoparticles (cRGDfK-conjugated camptothecin-loaded PGA-PA nanoparticles [RCPN]) were prepared and physico-chemically characterized using different techniques. Nanoparticles were evaluated for in vitro anticancer activity, cellular uptake, induction of apoptosis and wound healing cell migration against U87MG human glioblastoma cells.

Results: RCPN, with a particle size of < 100 nm and 65% CPT encapsulation efficiency, exhibited a dose-and time-dependent cytotoxicity to glioblastoma cells. Compared with native CPT or unconjugated nanoparticles, RCPN induced apoptosis, increased reactive oxygen species generation and inhibited U87MG cell migration.

Conclusion: cRGDfK-mediated and amphiphilic copolymer-based nanomedicines represent a new approach for improved delivery of anticancer drugs to and treatment of glioblastoma multiforme.