A rapid, maskless 3D prototyping for fabrication of capillary circuits: Toward urinary protein detection

Authors

Sheng Yan, University of WollongongFollow
Yuanqing Zhu, University of Science and Technology of China
Shiyang Tang, University of WollongongFollow
Yuxing Li, University of Wollongong
Qianbin Zhao, University of WollongongFollow
Dan Yuan, University of WollongongFollow
Guolin Yun, University of WollongongFollow
Jun Zhang, Nanjing University of Science And TechnologyFollow
Shiwu Zhang, University of Science and Technology of ChinaFollow
Weihua Li, University of WollongongFollow

RIS ID

119053

Publication Details

Yan, S., Zhu, Y., Tang, S., Li, Y., Zhao, Q., Yuan, D., Yun, G., Zhang, J., Zhang, S. & Li, W. (2018). A rapid, maskless 3D prototyping for fabrication of capillary circuits: Toward urinary protein detection. Electrophoresis, 39 (7), 957-964.

Abstract

Proteinuria is an established risk marker for progressive renal function loss and patients would significantly benefit from a point-of-care testing. Although extensive work has been done to develop the microfluidic devices for the detection of urinary protein, they need the complicated operation and bulky peripherals. Here, we present a rapid, maskless 3D prototyping for fabrication of capillary fluidic circuits using laser engraving. The capillary circuits can be fabricated in a short amount of time ( < 10 min) without the requirements of clean-room facilities and photomasks. The advanced capillary components (e.g., trigger valves, retention valves and retention bursting valves) were fabricated, enabling the sequential liquid delivery and sample-reagent mixing. With the integration of smartphone-based detection platform, the microfluidic device can quantify the urinary protein via a colorimetric analysis. By eliminating the bulky and expensive equipment, this smartphone-based detection platform is portable for on-site quantitative detection.