RIS ID
140139
Abstract
Human brain organoids provide opportunities to produce three-dimensional (3D) brain-like tissues for biomedical research and translational drug discovery, toxicology, and tissue replacement. Here we describe a protocol for rapid and defined induction of brain organoids from human induced pluripotent stem cells (iPSCs), using commercially available culture and differentiation media and a cheap, easy to handle and clinically approved semisynthetic hydrogel. Importantly, the methodology is uncomplicated, well-defined, and reliable for reproducible and scalable organoid generation, and amendable to principles of current good laboratory practice (cGLP), with the potential for prospective adaptation to current good manufacturing practice (cGMP) toward clinical compliance.
Grant Number
ARC/CE140100012
Publication Details
Tomaskovic-Crook, E. & Crook, J. M. (2019). Clinically amendable, defined, and rapid induction of human brain organoids from induced pluripotent stem cells. Organoids, 1576 13-22.