An integrated centrifugo-opto-microfluidic platform for arraying, analysis, identification and manipulation of individual cells
RIS ID
95736
Abstract
In this work we present a centrifugal microfluidic system enabling highly efficient collective trapping and alignment of particles such as microbeads and cells, their multi-colour fluorescent detection and subsequent manipulation by optical tweezers. We demonstrate array-based capture and imaging followed by "cherry-picking" of individual particles, first for fluorescently labelled polystyrene (PS) beads and then for cells. Different cell lines are discriminated based on intracellular as well as surface-based markers.
Publication Details
Burger, R., Kurzbuch, D., Gorkin, R., Kijanka, G., Glynn, M., McDonagh, C. & Ducree, J. (2015). An integrated centrifugo-opto-microfluidic platform for arraying, analysis, identification and manipulation of individual cells. Lab on a Chip: miniaturisation for chemistry, physics, biology, materials science and bioengineering, 15 (2), 378-381.