Immobilization of acetylcholinesterase in nanofibrous PVA/BSA membranes by electrospinning

Authors

Atousa Moradzadegan, Shahid Beheshti University
Seyed-Omid Ranaei-Siadat, Shahid Beheshti University
Azadeh Ebrahim-Habibi, Tehran University of Medical Sciences
Mohammad Barshan-Tashnizi, Shahid Beheshti University
Rouhollah Jalili, New Ideas Research Institute (NIRI)Follow
Seyed-Fakhraddin Torabi, University of Tehran
Khosro Khajeh, Tarbiat Modares University

RIS ID

92354

Publication Details

Moradzadegan, A., Ranaei-Siadat, S., Ebrahim-Habibi, A., Barshan-Tashnizi, M., Jalili, R., Torabi, S. & Khajeh, K. (2010). Immobilization of acetylcholinesterase in nanofibrous PVA/BSA membranes by electrospinning. Engineering in Life Sciences, 10 (1), 57-64.

Abstract

Electrospinning, a simple and versatile method to fabricate nanofibrous supports, has attracted continuous attention in the field of enzyme immobilization. In this study, acetylcholinesterase (AChE) has been successfully immobilized in PVA nanofibers via electrospinning of a mixture of AChE, BSA as an enzyme stabilizing additive and PVA. The maximum activity recovery of immobilized AChE was about 40%. In comparison with free enzyme, the immobilized AChE showed improved stability while retaining a considerable amount of activity at lower pH values. Moreover, the immobilized AChE retained >34% of its initial activity when stored at 30°C for 100 days and retained 70% of its initial activity after ten consecutive reactor batch cycles.