The therapeutic effects of an oleanolic acid derivative in diet induced obesity
Purpose: Recently, studies have shown that pentacyclic triterpenes have anti-inflammatory effects in the brains of mice with obesity; a disorder where inflammation largely contributes to energy balance dysregulation. The purpose of this study was to: 1) Investigate for the first time if the oleanolic acid (OA) derivative, bardoxolone methyl (BM) has a more potent effect than ursolic acid (UA) and OA in reducing body weight in mice fed a high fat diet (HFD) for 10 weeks; 2) To analyse hypothalamic tissue of mice in this study to determine if UA, OA and BM improve key neuronal molecules involved in energy expenditure. Methods: 12 week C57B1/6 male mice (n=70) were divided into 5 groups (low fat diet (LFD), HFD, HFD + UA, HFD + OA and HFD +BM). BM, UA and OA were administered in drinking water at a dosage of 10mg/kg. Signalling molecules involved in energy expenditure is being analysed using western blotting and Real time polymerase chain reaction (RTPCR). One and two way analysis of variance (ANOVA) and post-hoc Tukey-HSD test were performed for statistical analysis. Results: BM significantly prevented body weight gain in mice fed a HFD compared to the mice treated with OA and UA (p≤
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