Enhancing harvest of biodiesel-promising microalgae using Daphnia domesticated by amino acids

Publication Name

Environmental Research

Abstract

Mass production of microalgal biodiesel is hindered by microalgae harvesting efficiency and costs. In this study, Daphnia domesticated by amino acids were used to harvest microalgae via ingesting. The main factors (density of Daphnia, salinity, pH, light-environment, temperature and algal concentration) that were conducive to Daphnia feeding were optimized. Under the optimal condition, Microalgae-feeding Daphnia were domesticated by adding D-glutamic acid and L-cysteine as stimulating factors. After that, the ingestion rate of domesticated Daphnia increased by 24.93%. The presence of Daphnia as a predator can induce microalgae to mass into clusters. Combining Daphnia feeding and the inductive defense flocculation of microalgae, the harvesting rate of mixed algae (Chlorella pyrenoidosa and Scenedesmus obliquus) reached over 95% after 9 h. Overall, this work suggested that Daphnia feeding process is a green and economical approach for microalgae harvesting.

Open Access Status

This publication is not available as open access

Volume

212

Article Number

113465

Funding Number

GXWD20201230155427003-20200821174135002

Funding Sponsor

Natural Science Foundation of Shenzhen City

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Link to publisher version (DOI)

http://dx.doi.org/10.1016/j.envres.2022.113465