Making neurons, made easy: The use of Neurogenin-2 in neuronal differentiation
Publication Name
Stem Cell Reports
Abstract
Directed neuronal differentiation of human pluripotent stem cells (hPSCs), neural progenitors, or fibroblasts using transcription factors has allowed for the rapid and highly reproducible differentiation of mature and functional neurons. Exogenous expression of the transcription factor Neurogenin-2 (NGN2) has been widely used to generate different populations of neurons, which have been used in neurodevelopment studies, disease modeling, drug screening, and neuronal replacement therapies. Could NGN2 be a “one-glove-fits-all” approach for neuronal differentiations? This review summarizes the cellular roles of NGN2 and describes the applications and limitations of using NGN2 for the rapid and directed differentiation of neurons.
Open Access Status
This publication may be available as open access
Volume
17
Issue
1
First Page
14
Last Page
34
Funding Number
2007421
Funding Sponsor
Friedreich's Ataxia Research Alliance