Title
A method for topical dosing of invertebrates with pesticide for use in feeding experiments
Publication Name
Ecotoxicology
Abstract
The ability to produce large numbers of pesticide-exposed insects (e.g. crickets) is important for feeding studies into the effects of pesticides on key predatory species. House crickets (Acheta domesticus L. 1758) were submersed in serial dilutions of the pesticides, fenitrothion and fipronil, used for the control of locusts in Australia, and then rapidly frozen for residue analysis. Good correlations were found between increasing concentrations of serial pesticide dilutions and the resultant residual concentrations of the parent compounds in crickets, with R values of 0.949 (fenitrothion) and 0.946 (fipronil). R values for the much less abundant fipronil metabolites were lower 0.858 (sulfone), 0.368 (desulfinyl) and 0.785 (sulfide). This method enables insecticide exposure mimicking the field conditions to be assessed, and can be done immediately prior to an experiment. This ensures locusts remain alive when introduced to the feeding chambers, and enables multiple prey items to be dosed with a known pesticide burden. 2 2
Open Access Status
This publication is not available as open access
Volume
30
Issue
2
First Page
381
Last Page
386
Funding Number
LP160100686
Funding Sponsor
Australian Research Council