Title

Bioengineering clinically relevant cardiomyocytes and cardiac tissues from pluripotent stem cells

Publication Name

International Journal of Molecular Sciences

Abstract

The regenerative capacity of cardiomyocytes is insufficient to functionally recover damaged tissue, and as such, ischaemic heart disease forms the largest proportion of cardiovascular associated deaths. Human-induced pluripotent stem cells (hiPSCs) have enormous potential for developing patient specific cardiomyocytes for modelling heart disease, patient-based cardiac toxicity testing and potentially replacement therapy. However, traditional protocols for hiPSC-derived cardiomyocytes yield mixed populations of atrial, ventricular and nodal-like cells with immature cardiac properties. New insights gleaned from embryonic heart development have progressed the precise production of subtype-specific hiPSC-derived cardiomyocytes; however, their physiological immaturity severely limits their utility as model systems and their use for drug screening and cell therapy. The long-entrenched challenges in this field are being addressed by innovative bioengingeering technologies that incorporate biophysical, biochemical and more recently biomimetic electrical cues, with the latter having the potential to be used to both direct hiPSC differentiation and augment maturation and the function of derived cardiomyocytes and cardiac tissues by mimicking endogenous electric fields.

Open Access Status

This publication may be available as open access

Volume

22

Issue

6

Article Number

3005

First Page

1

Last Page

34

Funding Number

CE140100012

Funding Sponsor

Australian Research Council

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Link to publisher version (DOI)

http://dx.doi.org/10.3390/ijms22063005