This study aimed to examine food safety knowledge and its associations among Australians. An Internet-based nationwide survey of 2,022 consumers was conducted in 2011. Quota sampling was used to ensure that the age, gender, educational background, and state of residence of the respondents were representative of the Australian population. A list of 10 food safety knowledge items was administered along with questions about the respondents' food attitudes, demographics, school education, and diet practices. Overall, the results showed that safety knowledge was relatively poor. Latent class analysis identified two groups of respondents with different levels of food safety knowledge. Poor knowledge was negatively associated with age, the female gender, university education, experience of home economics or health education at school, the use of salt reduction diets, and general interest in food issues. The study's limitations and implications are discussed.