RIS ID

139736

Publication Details

Whinn, K. S., van Oijen, A. M. & Ghodke, H. (2019). Spy-ing on Cas9: Single-molecule tools reveal the enzymology of Cas9. Current Opinion in Biomedical Engineering, 12 25-33.

Abstract

Clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas) systems are an adaptive immune response mechanism in prokaryotes which can target and cleave invading DNA or RNA. The rapid understanding of the type II CRISPR/Cas9 system through biochemical, genetic and structural investigations has contributed to the development of Cas9 for various DNA- and RNA-targeting applications. Recent single-molecule investigations of CRISPR/Cas9 mechanisms have further extended our understanding of target search, binding and cleavage. These investigations are fundamental to the further development of CRISPR/Cas9 tools. This review discusses how single-molecule techniques have illuminated the enzymology of Cas9 endonucleases.

Grant Number

ARC/DP180100858

Grant Number

ARC/FL140100027

Available for download on Monday, September 27, 2021

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