Regional Sensitivity to Climate Change in Antarctic Terrestrial and Limnetic Ecosystems (RiSCC) is an international research program on Antarctic terrestrial and limnetic organisms and ecosystems. It is sponsored by the Scientific Committee on Antarctic Research (SCAR), a committee of the International Council of Science (ICSU). The RiSCC programme was formally adopted as a research programme under the auspices of the SCAR Working Group on Biology during SCAR XXVI, in Tokyo, Japan, July 2000. A recommendation to approve of this programme was made to the Delegates Meeting of SCAR XXVI. The Delegates agreed to this recommendation. In this manual the agreed methods are being described, which are to be used in RiSCC projects. Methods presented here have been tried in polar regions under extreme conditions, or with organisms obtained from high latitudes, and have been considered appropriate, providing reliable data.