Investigation of Group A Streptococcal Interactions with Host Glycan Structures Using High-Throughput Techniques: Glycan Microarray Analysis Using Recombinant Protein and Whole Cells

Authors

Anuk Indraratna, University of WollongongFollow
David De Oliveira, University of WollongongFollow
Lauren Hartley-Tassell
Christopher Day
Mark Walker
Michael Jennings
Martina L. Sanderson-Smith, University of WollongongFollow

RIS ID

143401

Publication Details

Indraratna, A., De Oliveira, D., Hartley-Tassell, L., Day, C., Walker, M., Jennings, M. & Sanderson-Smith, M. (2020). Investigation of Group A Streptococcal Interactions with Host Glycan Structures Using High-Throughput Techniques: Glycan Microarray Analysis Using Recombinant Protein and Whole Cells. Methods in Molecular Biology, 2136 145-151.

Abstract

Glycans, also known as carbohydrates, are abundant upon cell surfaces, where they often mediate host-pathogen interactions. The specific recognition of host glycans by pathogenic lectins is an important process that allows the adherence of bacteria to the host epithelial surface in many species, including Group A Streptococcus (GAS). Glycan microarrays present a sensitive, high-throughput approach for identifying novel lectin-glycan interactions and can be applied in the context of whole bacteria or purified bacterial proteins.