The mRNA-based reprogramming of fibroblasts from a SOD1^E101G familial amyotrophic lateral sclerosis patient to induced pluripotent stem cell line UOWi007

Authors

Rachelle Balez, University of WollongongFollow
Tracey Berg, University of WollongongFollow
Monique Bax, University of WollongongFollow
Sonia Sanz Munoz, University of Wollongong, University of WollongongFollow
Mauricio E Castro Cabral Da Silva, University of WollongongFollow
Martin Engel, University of WollongongFollow
Phuong Dzung Do-Ha, University of WollongongFollow
Claire H. Stevens, University of WollongongFollow
Dominic Rowe
Shu Yang
Ian Blair
Lezanne Ooi, University of WollongongFollow

RIS ID

141168

Publication Details

Balez, R., Berg, T., Bax, M., Sanz Munoz, S., E Castro Cabral Da Silva, M., Engel, M., Do-Ha, P., Stevens, C., Rowe, D., Yang, S., Blair, I. & Ooi, L. (2020). The mRNA-based reprogramming of fibroblasts from a SOD1^E101G familial amyotrophic lateral sclerosis patient to induced pluripotent stem cell line UOWi007. Stem Cell Research, 42

Abstract

2020 The Authors Dermal fibroblasts were donated by a 43 year old male patient with clinically diagnosed familial amyotrophic lateral sclerosis (ALS), carrying the SOD1E101G mutation. The induced pluripotent stem cell (iPSC) line UOWi007-A was generated using repeated mRNA transfections for pluripotency transcription factors Oct4, Klf4, Sox2, c-Myc, Lin28 and Nanog. The iPSCs carried the SOD1E101G genotype and had a normal karyotype, expressed expected pluripotency markers and were capable of in vitro differentiation into endodermal, mesodermal and ectodermal lineages. This iPSC line may be useful for investigating familial ALS resulting from a SOD1 E101G mutation.