The changing point-spread function: single-molecule-based super-resolution imaging

Authors

Mathew H. Horrocks, University of CambridgeFollow
Matthieu Palayret, University of Cambridge
David Klenerman, University of Cambridge
Steven F. Lee, University of Cambridge

RIS ID

104341

Publication Details

Horrocks, M. H., Palayret, M., Klenerman, D. & Lee, S. F. (2014). The changing point-spread function: single-molecule-based super-resolution imaging. Histochemistry and Cell Biology, 141 (6), 577-585.

Abstract

Over the past decade, many techniques for imaging systems at a resolution greater than the diffraction limit have been developed. These methods have allowed systems previously inaccessible to fluorescence microscopy to be studied and biological problems to be solved in the condensed phase. This brief review explains the basic principles of super-resolution imaging in both two and three dimensions, summarizes recent developments, and gives examples of how these techniques have been used to study complex biological systems.