Incorporation of chlorinated analogues of aliphatic amino acids during cell-free protein synthesis

Authors

Dannon J. Stigers, Australian National University
Zachary I. Watts, Australian National University
James E. Hennessy, Australian National University
Hye-Kyung Kim, Australian National University
Romeo Martini, Australian National University
Matthew C. Taylor, CSIRO Entomology
Kiyoshi Ozawa, University of WollongongFollow
Jeffrey W. Keillor, Australian National University
Nicholas E. Dixon, University of WollongongFollow
Christopher J. Easton, Australian National University

RIS ID

35338

Publication Details

Stigers, D. J., Watts, Z. I., Hennessy, J. E., Kim, H., Martini, R., Taylor, M. C., Ozawa, K., Keillor, J. W., Dixon, N. E. & Easton, C. (2011). Incorporation of chlorinated analogues of aliphatic amino acids during cell-free protein synthesis. Chemical Communications, 47 (6), 1839-1841.

Abstract

3-Chloro-Abu and 4-chloro-Nva are biosynthetically incorporated into E. coli peptidyl-Pro cis-trans isomerase B, as substitutes for Val and Leu, respectively. The extent of incorporation is up to 90%, and substituted protein is catalytically active. By contrast, 4-chloro-Val is not an effective replacement for Ile.