Title
Cell-free transcription/translation from PCR-amplified DNA for high-throughput NMR studies
RIS ID
16854
Abstract
Quick and clean: Excellent protein yields in cell-free protein synthesis can be obtained by using polymerase chain reaction (PCR) amplified DNA templates, provided that the templates are designed for cyclization. This eliminates time-consuming cloning steps and allows one to go from complementary DNA to the protein NMR spectrum in 24 h.
Publication Details
Wu, P. S.C., Ozawa, K., Lim, S. P., Vasudevan, S. G., Dixon, N. E. and Otting, G. (2007). Cell-free transcription/translation from PCR-amplified DNA for high-throughput NMR studies. Angewandte Chemie (International Edition), 46 (18), 3356-3358.