Cell-free transcription/translation from PCR-amplified DNA for high-throughput NMR studies

Authors

Peter S.C. Wu, Australian National University
Kiyoshi Ozawa, University of WollongongFollow
Siew P. Lim, Novartis Institute for Tropical Diseases Pte Ltd
Subhash G. Vasudevan, Novartis Institute for Tropical Diseases Pte Ltd
Nicholas E. Dixon, University of WollongongFollow
Gottfried Otting, Australian National University

RIS ID

16854

Publication Details

Wu, P. S.C., Ozawa, K., Lim, S. P., Vasudevan, S. G., Dixon, N. E. and Otting, G. (2007). Cell-free transcription/translation from PCR-amplified DNA for high-throughput NMR studies. Angewandte Chemie (International Edition), 46 (18), 3356-3358.

Abstract

Quick and clean: Excellent protein yields in cell-free protein synthesis can be obtained by using polymerase chain reaction (PCR) amplified DNA templates, provided that the templates are designed for cyclization. This eliminates time-consuming cloning steps and allows one to go from complementary DNA to the protein NMR spectrum in 24 h.