Unsuccessful ageing: dramatic changes to the human lens lipidome with age
It has been postulated that organelle degradation and loss of metabolic activity in the lens centre result in a lack of protein or lipid turnover. This would have major implications for lens function with age. Previous work into age-related lipid alterations has involved pooling whole human lenses. However, for an accurate comparison of age-related lipid alterations, it is necessary to analyse the lens present from birth, i.e. the nucleus. Accordingly, the aim of this study was to analyse lipid changes to individual human lens nuclear regions with age, and map the lens lipid distribution using highly sensitive analytical tools.