Effect of escherichia coli lipopolysaccharide on stretch-induced ATP release from cultured urothelial cells
Urinary tract infection (UTI), commonly caused by Escherichia coli, is experienced by 50% of all women at least once during their lifetime. The symptoms of UTI include increased voiding frequency and urgency, usually accompanied by a sensation of burning pain and foul smelling urine. ATP released from the bladder urothelium is an important molecule for signaling urinary urgency. Lipopolysaccharide (LPS) is the major component of the outer membrane of Gram-negative bacteria and triggers the induction of host immune responses. LPS is made up of a highly conserved core and lipid A together with a surface-exposed O antigen. There are over 180 different O antigen types produced by different E. coli strains. The aim of the current study was to examine the effect of LPS from E. coli on stretch-induced ATP release from human urothelial cells in culture.