Separation and identification of phosphatidylcholine regioisomers by combining liquid chromatography with a fusion of collision-and ozone-induced dissociation

Authors

Rachel Kozlowski, University of WollongongFollow
Todd W. Mitchell, University of WollongongFollow
Stephen J. Blanksby, University of WollongongFollow

RIS ID

104795

Publication Details

Kozlowski, R. L., Mitchell, T. W. & Blanksby, S. J. (2015). Separation and identification of phosphatidylcholine regioisomers by combining liquid chromatography with a fusion of collision-and ozone-induced dissociation. European Journal of Mass Spectrometry, 21 (3), 191-200.

Abstract

The differentiation of closely related lipid isomers is increasingly important to our evolving understanding of lipid biochemistry but it is equally challenging to contemporary chromatographic and mass spectral analyses. Recently, we described a novel ion-activation approach based on combining collision- with ozone-induced dissociation (CID/OzID) for the identification of the relative acyl chain substitution positions in glycerophospholipids. Here we demonstrate, for the first time, that CID/OzID can be effectively combined with reversed-phase chromatography to enable the separation and unambiguous identification of regioisomeric pairs of phosphatidylcholines that differ only in the arrangement of acyl chains on the glycerol backbone.

Grant Number

ARC/FT110100249, ARC/DP120102922, ARC/LP110200648

Additional Grant Number

http://purl.org/au-research/grants/ARC/FT110100249

http://purl.org/au-research/grants/ARC/DP120102922

http://purl.org/au-research/grants/ARC/LP110200648