Title

Generation of Neural Crest Progenitors from Human Pluripotent Stem Cells

RIS ID

135297

Publication Details

Abu-Bonsrah, K., Viventi, S., Newgreen, D. F. & Dottori, M. (2019). Generation of Neural Crest Progenitors from Human Pluripotent Stem Cells. Methods in Molecular Biology, 1976 37-47.

Abstract

There are a vast range of diseases and disorders that are neurocristopathic in origin, including Hirschsprung's disease, pheochromocytoma, familial dysautonomia, craniofacial disorders, and melanomas. Having a source of human neural crest cells is highly valuable for investigating potential treatments for such diseases. This chapter describes a robust and well-characterized protocol for deriving neural crest from human pluripotent stem cells (hPSCs), which can then be differentiated to neuronal and non-neuronal lineages. The protocol is adapted to suit hPSC maintenance as a monolayer bulk culture or as manual-passaged colonies, which makes it widely applicable to researchers that may use different systems for hPSC maintenance.

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Link to publisher version (DOI)

http://dx.doi.org/10.1007/978-1-4939-9412-0_3