Viral-free generation and characterization of a human induced pluripotent stem cell line from dermal fibroblasts

Authors

Martin Engel, University of WollongongFollow
Rachelle Balez, University of WollongongFollow
Sonia Sanz Munoz, University of WollongongFollow
Mauricio E Castro Cabral Da Silva, University of WollongongFollow
Claire H. Stevens, University of WollongongFollow
Monique Bax, University of WollongongFollow
Phuong Dzung Do-Ha, University of WollongongFollow
Kuldip Sidhu, University of New South Wales
Perminder Sachdev, University of New South Wales
Lezanne Ooi, University of WollongongFollow

RIS ID

130693

Publication Details

Engel, M., Balez, R., Sanz Munoz, S., Cabral-da-Silva, M. Castro., Stevens, C. Helen., Bax, M., Do-Ha, D., Sidhu, K., Sachdev, P. & Ooi, L. (2018). Viral-free generation and characterization of a human induced pluripotent stem cell line from dermal fibroblasts. Stem Cell Research, 32 135-138.

Abstract

Peripheral dermal fibroblasts (DF) from a healthy 56 year old female were obtained from the Centre for Healthy Brain Ageing (CHeBA) Biobank, University of New South Wales, under the material transfer agreement with the University of Wollongong. DFs were reprogrammed via mRNA-delivered transcription factors into induced pluripotent stem cells (iPSCs). The generated iPSCs were confirmed to be pluripotent, capable of three germ layer differentiation and are thus a useful resource for creating iPSC-derived healthy human cells of any lineage.

Grant Number

NHMRC/1135720

Grant Number

NHMRC/1079995

Additional Grant Number

http://purl.org/au-research/grants/NHMRC/1071250