An experimental method was developed to study the adherence properties of the oxide scale formed on microalloyed low carbon steel after hot strip rolling. The evolution of the oxide scale during laminar cooling was investigated using Gleeble 3500 Thermal-Mechanical Simulator connected with a humid air generator. After the sample cooled down to ambient temperature, the oxide scale was protected by lacquer to prevent the scale from losing. Physicochemical characteristics of the oxide scale were examined and the adherence mechanism was discussed. Decomposed wustite a mixture of α-iron and magnetite (Fe3O4), can substantially improve the integrity of oxide scale. However, large quantities of hematite (Fe2O3) or retained wustite (FeO) were found detrimental to the adhesion of the oxide scale. It is found that the adherence of oxide scales significantly depends on the phase composition of oxide scales with different thickness.