© 2020 American Society of Civil Engineers. Column experiments that investigate the use of calcitic limestone as a potential material for permeable reactive barriers (PRBs), as well as its clogging behavior, are conducted under conditions that involve continuous acidic flow containing Al, Fe, and acidophilic bacteria. Results show that nonhomogenous biogeochemical clogging occurred toward the outlet, resulting in a 45% reduction of hydraulic conductivity at the inlet and 10% reduction at the outlet after the bicarbonate buffering period. A mathematical model developed to capture the reductions in longevity is presented. The model, which considers the effects of time-varying porosity, hydraulic conductivity, and head at a particular point on the horizontal flow path, is used for assessing the effect of coupled clogging in a calcitic porous medium.