[Effects of hippocampal stimulus on α₅ subunit of extrasynaptic GABA(A) receptor in kainic acid-induced epileptic rats]
RIS ID
104966
Abstract
METHODS: A total of 40 healthy male Sprague Dawley (SD) rats were randomly divided into 5 groups of epilepsy, sham operation, low-frequency stimulation (LFS), high-frequency stimulation (HFS) and normal control (n = 8 each). The expression of α₅ subunit of extrasynaptic GABA(A) receptor as well as the association with the curative effects of LFS were evaluated by behavioristics, real-time fluorogenic quantitative polymerase chain reaction (PCR) and Western blot. RESULTS: The kainic acid kindling rats had class V attack with a modeling success rate of 67%. After treatment, as compared with sham operation and epilepsy groups, the number of seizures decreased significantly in LFS and HFS treatment groups (P < 0.01). But LFS and HFS treatment groups were compared, the number of seizures has no statistical significance (P > 0.05). When sham operation and epilepsy groups were compared, the number of seizures was not statistically significant (P > 0.05). The paired inter-group comparisons showed that LFS and HFS treatment groups and sham operation and epilepsy groups had significant differences in α₅ subunit mRNA and protein (P < 0.01). No statistical significance existed between LFS and HFS treatment groups (P > 0.05) or sham operation and HFS treatment groups (P > 0.05). As compared with normal control group, LFS treatment and HFS groups had significant difference (P < 0.01). CONCLUSION: Electrical hippocampal stimulation can effectively inhibit seizures. Both LFS and HFS are equally effective, but the former offers greater benefits. And an inhibition of extrasynaptic α₅ subunit plays an important role in seizure during hippocampal stimulation. OBJECTIVE: To observe the effects of electrical hippocampal stimulation of α₅ subunit of extrasynaptic GABA(A) receptor in kainic acid-induced epileptic rats, explore the optimal therapeutic parameters and elucidate the possible mechanism of electrical stimulation for hippocampal epilepsy.
Publication Details
Shen, F., Hui, L., Zhou, X., Wang, Z., Zhao, X., Zhou, W., Zhang, X., Yang, G., Wang, F. & Sun, T. (2014). [Effects of hippocampal stimulus on α₅ subunit of extrasynaptic GABA(A) receptor in kainic acid-induced epileptic rats]. Zhonghua yi xue za zhi, 94 (23), 1820-1824.