A hybrid dielectrophoretic and hydrophoretic microchip for particle sorting using integrated pre-focusing and sorting steps

Authors

Sheng Yan, University of WollongongFollow
Jun Zhang, University of WollongongFollow
Yuan Yuan, University of New South Wales
George Lovrecz, CSIRO Manufacturing Flagship
Gursel Alici, University of WollongongFollow
Haiping Du, University of WollongongFollow
Yonggang Zhu, Csiro Materials Science And Engineering, CSIRO Manufacturing Flagship
Weihua Li, University of WollongongFollow

RIS ID

96114

Publication Details

Yan, S., Zhang, J., Yuan, Y., Lovrecz, G., Alici, G., Du, H., Zhu, Y. & Li, W. (2015). A hybrid dielectrophoretic and hydrophoretic microchip for particle sorting using integrated pre-focusing and sorting steps. Electrophoresis, 36 (2), 284-291.

Abstract

This work explores dielectrophoresis (DEP)-active hydrophoresis in sorting particles and cells. The device consists of prefocusing region and sorting region with great potential to be integrated into advanced lab-on-a-chip bioanalysis devices. Particles or cells can be focused in the prefocusing region and then sorted in the sorting region. The DEP-active hydrophoretic sorting is not only based on size but also on dielectric properties of the particles or cells of interest without any labelling. A mixture of 3 and 10 um particles were sorted and collected from corresponding outlets with high separation efficiency. According to the different dielectric properties of viable and nonviable Chinese Hamster Ovary (CHO) cells at the medium conductivity of 0.03 S/m, the viable CHO cells were focused well and sorted from cell sample with a high purity.