In this study, the Plaque SimulatorTM eye plaque brachytherapy planning system was commissioned for ROPES eye plaques and Amersham Health model 6711 Iodine 125 seeds, using TG43-UI data. The brachytherapy module of the RADCALC® independent checking program was configured to allow verification of the accuracy of the dose calculated by Plaque SimulatorTM. Central axis depth dose distributions were compared and observed to agree to within 2% for all ROPES plaque models and depths of interest. Experimental measurements were performed with a customized PRESAGEm 3-D type dosimeter to validate the calculated depth dose distributions. Preliminary results have shown the effect of the stainless steel plaque backing decreases the measured fluorescence intensity by up to 25%, and 40% for the 15 mm and 10 mm diameter ROPES plaques respectively. This effect, once fully quantified should be accounted for in the Plaque SimulatorTM eye plaque brachytherapy planning system.