The determination of steric purity of amines and amino acids gas chromatography and mass spectrometry

Amines and amino acids have been condensed with a mixture of N-TFA-R-prolyl chloride and N-TFA-S-(1-^H)-prolyl chloride and the resulting four diastereoisomers have been separated by Gas Chromatography into two peaks. The amount of each diastereoisomer formed was then determined by Chemical Ionisation Mass Spectrometry. The steric purity of the enantiomeric mixtures was calcuated from these values and the calculated values were always found to be within 5% of the expected values.